KMID : 0620920170490090009
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Experimental & Molecular Medicine 2017 Volume.49 No. 9 p.9 ~ p.9
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TGF-¥â1 suppresses CCL3/4 expression through the ERK signaling pathway and inhibits intervertebral disc degeneration and inflammation-related pain in a rat model
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Zhang Jian
Li Ze Min Chen Fan Liu Hui Wang Hua Li Xiang Liu Xian Guo Wang Jian Ru Zheng Zhao Min
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Abstract
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The objective of this study was to investigate the regulatory effects of TGF-¥â1 on CCL3/4 expression and inflammation-related pain during intervertebral disc degeneration (IVDD). TGF-¥â1 and CCL3/4 expression patterns in different degenerative human nucleus pulposus (NP) tissues were measured by qPCR and immunohistochemistry (IHC), and the effects of TGF-¥â1 on CCL3/4 expression were measured by qPCR, ELISA and immunofluorescence. The roles of NF-¥êB and MAPK in TGF-¥â1-mediated CCL3/4 promoter activity were studied using siRNAs, western blotting and qPCR. After establishing an IVDD rat model in vivo, we administered intradiscal injections of TGF-¥â1. The effects of TGF-¥â1 on IVDD were determined by MRI and histological analyses, and the effects of TGF-¥â1 on dorsal root ganglion (DRG) inflammation and pain development were determined by IHC staining and pain-behavior testing, respectively. TGF-¥â1 and CCL3/4 expression was elevated in degenerative NP tissue. CCL4 expression was significantly inhibited by TGF-¥â1 treatment. Pharmacological inhibition or siRNA knockdown of the ERK1/2 signaling attenuated TGF-¥â1-mediated suppression of CCL4 expression. In vivo, TGF-¥â1 injection inhibited the development of degenerative features in the IVDD model. Moreover, TGF-¥â1 prevented the inflammatory response and pain development. The results of this study show that TGF-¥â1 downregulates CCL4 expression through ERK1/2 signaling activation in NP cells. Furthermore, TGF-¥â1 can prevent degenerative processes, inhibit inflammatory responses in the DRG and prevent pain development in the IVDD rat model. The results of this study indicate that TGF-¥â1 may represent a therapeutic target for the control of inflammation-related pain associated with IVDD.
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KEYWORD
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Extracellular signalling molecules
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